Analysis Note

Positive ControlA-431 cells

Negative ControlHL-60 cells

Application

Flow Cytometry (1-5 g/ml)Immunofluorescence (1-5 g/ml)Immunoprecipitation (1-2 g/sample)Neutralization Studies (1-2 g/ml; see application references)

General description

Recognizes the ~170 kDa EGF receptor protein in A431 cells.

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Immunogen

partially purified, human EGFR from A431 cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hunter T., 1984. Nature311, 414.Reynolds, F.H., Jr., et al. 1981. Nature292, 259.Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Warning

Toxicity: Standard Handling (A)